Yes. But bear in mind that S&T will only deal with one route, or in your case one segment, at a time. This is how you would do it.
1 - Import the coordinates into S&T, I assume you know how to do that.
2 - Open a new completely blank file, without closing your first file, and arrange them as side-by-side windows on your desktop.
3 - Go to the Legend and overview panel of the file that has the coordinates and pick a pair of coordinates representing the start and end of a segment of interest and copy those onto the virgin file window. You can click and drag them or do ctrl-c
and ctrl-v. No precision is required when pasting - S&T will drop them to the 'right' place
4 - Now you will have two pushpins on your newer map. Go to the Legend and overview of that map and click on the new pushpin sets ("My pushpins") and right-click on it. Select "Add pushpins as stops" and click.
5 - Now go to the Route planner panel and you should see two stops (and only two stops - one after a green box and one after a red box). Click get directions and it will connect the dots.
6 - You can now print that (zooming in to whatever level you prefer) or copy it.
