w3wp: the server is not operational?

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  Hello - The CRM implementation I was working on was working in prod a few days ago but now it's returning an "unexpected error."  I've included the error written to the Windows Application log below.  We already tried the PreferredDc solution that seems to have worked for a lot of other people. However, it's not working for us. Can you make any recommendations on next steps?
  
  ============================================================
  
  Event code: 3005 
  Event message: An unhandled exception has occurred. 
  Event time: 8/16/2013 6:08:32 PM 
  Event time (UTC): 8/17/2013 1:08:32 AM 
  Event ID: 78fd6c1c783842879deb76918c46a83c 
  Event sequence: 823 
  Event occurrence: 3 
  Event detail code: 0 
   
  Application information: 
      Application domain: /LM/W3SVC/1/ROOT-1-130211749785727500 
      Trust level: Full 
      Application Virtual Path: / 
      Application Path: C:\Program Files\Microsoft Dynamics CRM\CRMWeb\ 
      Machine name: MSRC-CRM-PROD1 
   
  Process information: 
      Process ID: 3512 
      Process name: w3wp.exe 
      Account name: MYDOMAIN\SRCAUTO 
   
  Exception information: 
      Exception type: COMException 
      Exception message: The server is not operational.
  
     at System.DirectoryServices.DirectoryEntry.Bind(Boolean throwIfFail)
     at System.DirectoryServices.DirectoryEntry.Bind()
     at System.DirectoryServices.DirectoryEntry.get_NativeObject()
     at System.DirectoryServices.DirectoryEntry.Invoke(String methodName, Object[] args)
     at Microsoft.Crm.SecurityUtils.CheckMembership(Guid principalId, Guid groupId)
     at Microsoft.Crm.Caching.OrganizationSettingsCacheLoader.LoadCacheData(Guid key, ExecutionContext context)
  
   
   
  Request information: 
      Request URL: http://msrc-crm-prod1/MSRC/default.aspx 
      Request path: /MSRC/default.aspx 
      User host address: fe80::3963:c395:c7f0:3151/ 
      User: MYDOMAIN\v-ancicc 
      Is authenticated: True 
      Authentication Type: Negotiate 
      Thread account name: MYDOMAIN\SRCAUTO 
   
  Thread information: 
      Thread ID: 10 
      Thread account name: MYDOMAIN\SRCAUTO 
      Is impersonating: False 
      Stack trace:    at System.DirectoryServices.DirectoryEntry.Bind(Boolean throwIfFail)
     at System.DirectoryServices.DirectoryEntry.Bind()
     at System.DirectoryServices.DirectoryEntry.get_NativeObject()
     at System.DirectoryServices.DirectoryEntry.Invoke(String methodName, Object[] args)
     at Microsoft.Crm.SecurityUtils.CheckMembership(Guid principalId, Guid groupId)
     at Microsoft.Crm.Caching.OrganizationSettingsCacheLoader.LoadCacheData(Guid key, ExecutionContext context)
   
   
  Custom event details: 
  

  Saturday, August 17, 2013 1:17 AM