Not if you intend on using the Owner Lookup field to assign records. You would have to add a new System User Lookup to the form and apply the filter to that. Once you set this you could then perform the action of assigning programatically or via
workflow upon saving the form for example. There are a number of ways to implement a new feature like this, it sjust depends on how your users will accept it.
If you are just trying to prevent users from assigned to the wrong team or group of users then you could create a pre stage plugin against the assign message, validate the assign is valid and handle it this way. If you want to filter the list of users in
the lookup then you will need an alternative like I've already mentioned.
Don't forget there is also an Assign button in the Ribbon... If you create your own feature then you will need to remove this button.
Filter in Assign button CRM 2011
